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Microsatellite Loci Polymorphism in Miguschova Wheat and Common Wheat Cultivars
(2020) Tytenko, Natalia; Iefimenko, Tetiana; Antonyuk, Maksym; Navalikhina, Anastasiia; Shpylchyn, Vitalii; Martynenko, Viktoriia; Pasichnyk, Tetiana
Fusarium head blight is one of the most widespread and dangerous wheat diseases worldwide. Resistance to Fusarium is controlled by some main genes from different Triticinae genomes; however, common wheat has few such resistance genes. Miguschova wheat (Triticum miguschovae Zhir.) with Ab Ab GGDD genome is characterized by the genotype resistant to Fusarium head blight. In order to effectively use it as a source of introgressions to common wheat genome, molecular genetic polymorphisms should be identified, which could later be used for identification of Miguschova wheat introgressions in the common wheat genome. Microsatellite PCR analysis using primers to SSR loci with a known chromosome localization for common wheat identified 14 highly informative loci with specific to Miguschova wheat amplicons, localized on chromosomes of 6 homoeological groups. Seven other SSR loci were identified to have a limited informative value, as DNA of Miguschova wheat did not form any specific PCR product with corresponding primers (null allele). The informative value of those loci was limited to differentiation of wheat cultivars.