The spectral manifistation of the new luminescent styryl dyes photostability and phototoxic influence on the DNA
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Date
2006
Authors
Kudrya, V.
Yashchuk, V.
Gusak, V. V.
Losytskyy, M.
Kryvorotenko, D.
Balanda, A.
Yarmoluk, S.
Gumenjuk, Y.
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Journal ISSN
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Abstract
The spectral investigation methods of the phenomena of luminescent dye probes photostability and
phototoxic influence on the DNA were proposed. The optical absorption, fluorescence and phosphorescence
spectra of the samples of the newest investigated styryl dyes and the systems DNA +dye were studied.
The optical absorption spectra of the samples of these compounds were measured under the irradiation
of these samples by visible light. The results of the investigations carried out on a number of dyes were
analyzed and discussed. The changes of optical density D value in wavelength regions 250+300 nm
(that corresponds to the DNA first electronic transition) and 370+650 nm (that corresponds to a dye
electronic transition) of the DNA+dye solutions were fixed. The dynamics ofD(t) was not monotonous.
It was shown the Mn-Styr and Di-Styr-30 dyes are photochemically safe for the DNA; these dyes bound
to the DNA are more photostable than in free state. The Di-Styr-24 and Dst-MdO dyes show slight phototoxic
effect on the DNA. The versions of possible phototoxicity (and photostability) mechanisms are
proposed.
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Keywords
luminescent dyes, phototoxicity for the DNA, photostability, optical absorption spectrum, методи спектральних досліджень, фотостабільність, ДНК, фототоксичний вплив
Citation
The spectral manifistation of the new luminescent styryl dyes photostability and phototoxic influence on the DNA/ V. Yu. Kudrya, V. M. Yashchuk, V. V. Gusak, M. Yu. Losytskyy, D. V. Kryvorotenko, A. O. Balanda, S. M. Yarmoluk, Ya. O. Gumenjuk // Наукові записки НаУКМА. - 2006. - Т. 51 : Фізико-математичні науки. - С. 35-42.