Analysis of Arabidopsis non-reference accessions reveals high diversity of metabolic gene clusters and discovers new candidate cluster members

Simple item page
dc.contributor.authorMarszalek-Zenczak, Malgorzata
dc.contributor.authorSatyr, Anastasiia
dc.contributor.authorWojciechowski, Pawel
dc.contributor.authorZenczak, Michal
dc.contributor.authorSobieszczanska, Paula
dc.contributor.authorBrzezinski, Krzysztof
dc.contributor.authorIefimenko, Tetiana
dc.contributor.authorFiglerowicz, Marek
dc.contributor.authorZmienko, Agnieszka
dc.date.accessioned2023-02-20T12:26:27Z
dc.date.available2023-02-20T12:26:27Z
dc.date.issued2023
dc.description.abstractMetabolic gene clusters (MGCs) are groups of genes involved in a common biosynthetic pathway. They are frequently formed in dynamic chromosomal regions, which may lead to intraspecies variation and cause phenotypic diversity. We examined copy number variations (CNVs) in four Arabidopsis thaliana MGCs in over one thousand accessions with experimental and bioinformatic approaches. Tirucalladienol and marneral gene clusters showed little variation, and the latter was fixed in the population. Thalianol and especially arabidiol/baruol gene clusters displayed substantial diversity. The compact version of the thalianol gene cluster was predominant and more conserved than the noncontiguous version. In the arabidiol/baruol cluster, we found a large genomic insertion containing divergent duplicates of the CYP705A2 and BARS1 genes. The BARS1 paralog, which we named BARS2, encoded a novel oxidosqualene synthase. The expression of the entire arabidiol/baruol gene cluster was altered in the accessions with the duplication. Moreover, they presented different root growth dynamics and were associated with warmer climates compared to the reference-like accessions. In the entire genome, paired genes encoding terpene synthases and cytochrome P450 oxidases were more variable than their nonpaired counterparts. Our study highlights the role of dynamically evolving MGCs in plant adaptation and phenotypic diversity.en_US
dc.identifier.citationAnalysis of Arabidopsis non-reference accessions reveals high diversity of metabolic gene clusters and discovers new candidate cluster members / Malgorzata Marszalek-Zenczak, Anastasiia Satyr, Pawel Wojciechowski, Michal Zenczak, Paula Sobieszczanska, Krzysztof Brzezinski, Tetiana Iefimenko, Marek Figlerowicz, Agnieszka Zmienko // Frontiers in Plant Science. - 2023. - Vol. 14. - Article no. 1104303. - https://doi.org/10.3389/fpls.2023.1104303en_US
dc.identifier.urihttps://doi.org/10.3389/fpls.2023.1104303
dc.identifier.urihttps://ekmair.ukma.edu.ua/handle/123456789/24716
dc.language.isoenuk_UA
dc.relation.sourceFrontiers in Plant Scienceen_US
dc.statusfirst publisheduk_UA
dc.subjectcopy number variationen_US
dc.subjectbiosynthetic gene clusteren_US
dc.subjectsecondary metabolismen_US
dc.subjectoxidosqualene cyclaseen_US
dc.subjecttriterpenesen_US
dc.subjectcytochrome P450en_US
dc.subjectarticleen_US
dc.titleAnalysis of Arabidopsis non-reference accessions reveals high diversity of metabolic gene clusters and discovers new candidate cluster membersen_US
dc.typeArticleuk_UA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Analysis_of_Arabidopsis_non-reference_accessions_reveals_high_diversity_of_metabolic_gene_clusters_and_discovers_new_candidate_cluster_members.pdf
Size:
5.62 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
7.54 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Кафедра біології