Кафедра лабораторної діагностики біологічних систем

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Browsing Кафедра лабораторної діагностики біологічних систем by Author "Didan, A."

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    Heparin-binding EGF-like growth factor as a prospective mediator of tissue repair and regeneration
    (2024) Didan, A.; Siromolot, Andriy; Kolybo, Denys
    Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the EGF family. It contains EGF-like and heparin-binding domains allowing interactions with heparin and cell-surface heparin sulfate. [1] It can activate human epidermal growth factor receptors 1 and 4 resulting in a multitude of cell responses, including cellular proliferation, migration, adhesion, and differentiation. HB-EGF levels can increase in response to different forms of injuries as well as extracellular stimuli, such as lysophosphatidic acid, retinoic acid, and 17-estradiol [2]. HB-EGF plays a critical role in tissue repair and regeneration. It promotes cutaneous wound healing, hepatocyte proliferation after partial hepatectomy, intestinal anastomosis strength, alveolar regeneration after pneumonectomy, neurogenesis after ischemic injury, bladder wall thickening in response to urinary tract obstruction, and protects against ischemia/reperfusion injury in many cell types [1, 3, 4]. Aim. To obtain bioactive recombinant human HB-EGF and investigate its proliferation capacity.
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    Production and in vitro Evaluation of Recombinant Human HB-EGF for Wound Healing and Targeted Therapy
    (2025) Vovk, I.; Didan, A.; Zhukova, Dariia; Dronko, Liliia; Rebriev, Andrew; Rybalko, A.; Legach, E.; Gorbatiuk, Oksana; Usenko, Mariia; Skvarchynskyi, Andrii; Dovbymchuk, T.; Siromolot, Andriy; Romaniuk, Svitlana; Kolybo, Denys
    Aim. The goal of the study was to evaluate the biological activity of recombinant human heparinbinding EGF-like growth factor (rhHB-EGF) on mouse fibroblasts in vitro and the possibility of its use as a potential agent for promoting wound healing and tissue regeneration. Methods. The study employed a scratch assay to evaluate the migration of mouse fibroblasts (L929 and NIH-3T3), the MTT test to assess cell proliferation and/or metabolic activity, MALDI-TOF mass spectrometry for protein identification, and flow cytometry to determine cell viability. Results. In the concentration range of 500–1000 ng/ml rhHB-EGF, no cytotoxic effect was recorded, but an increase in proliferation and/or metabolic activity, as well as migration of fibroblasts, was detected, with a maximum effect at 500 ng/ml rhHB-EGF in the cell incubation medium. A 30% overgrowth of the wound surface of fibroblasts was demonstrated in the scratch assay test under the influence of rhHB-EGF compared to the corresponding control. Conclusions. rhHB-EGF at a concentration of 500 ng/ml can be used in preparations to stimulate wound healing and tissue regeneration due to its ability to stimulate proliferation/metabolic activity and migration of fibroblasts, as well as the lack of cytotoxicity. Further, in vivo studies are needed for a comprehensive evaluation of this possibility.